Legionella is traditionally detected by culture on buffered charcoal yeast extract agar. Legionella requires the presence of cysteine and iron to grow, so does not grow on common blood agar media used for laboratory-based total viable counts or on-site dipslides.
BCYE Agar (Buffered Charcoal Yeast Extract), for Legionella, 15x100mm plate, order by the package of 10, by Hardy Diagnostics: Buffered Charcoal Yeast Extract (BCYE) Agar formulations are recommended for use in the cultivation and primary isolation of Legionella spp. in water and other samples suspected of harboring the bacteria. We compared the growth of 28 Legionella spp. on four manufacturers' buffered charcoal-yeast extract (BCYE) agar media and selective BCYE media that contained polymyxin B, anisomycin, and vancomycin or cefamandole. With BCYE as a "gold standard," growth a. buffered charcoal yeast extract b. MacConkey c. Hektoen Enteric (HE) d. Xylose-lysine Deoxycholate. Klebsiella pneumonia. The primary (true) pathogens of the Charcoal Yeast Extract Agar for the isolation of Legionellaceae when used with Legionella BCYE Growth Supplement SR0110 (Edelstein BYCE Agar). Format 10x90mm. Follow the link for more information on this medium. Only available in certain territories. Please speak to your local Oxoid products supplier.
Charcoal Yeast Extract Agar for the isolation of Legionellaceae when used with Legionella BCYE Growth Supplement SR0110 (Edelstein BYCE Agar). Format 10x90mm. Follow the link for more information on this medium. Only available in certain territories. Please speak to your local Oxoid products supplier.
The recovery of Legionella micdadei and L. bozemanii serogroups 1 and 2 from infected guinea pig spleens was evaluated by using two culture media: buffered charcoal yeast extract agar with 0.1% alpha-ketoglutarate (BCYE alpha) and the same medium supplemented with 1% bovine serum albumin (ABCYE alpha). At the lowest dilution of spleen tissue (10(-1)), recovery of all strains of L. micdadei and BCYE (Buffered Charcoal Yeast Extract) Selective Agar with GVPC antibiotics, for Legionella, 26ml fill, 15x100mm plate (Buffered Charcoal Yeast Extract Agar with Glycine, Vancomycin, Polymyxin, and Cycloheximide) For the selective isolation of Legionella spp A patient with chronic osteomyelitis caused by Brucella abortus had negative agglutination titers. Because of a superimposed staphylococcal infection that resulted in the overgrowth of this organism on nonselective media, brucellae were isolated only on a selective buffered charcoal-yeast extract (BCYE) agar.
BCYE (Buffered Charcoal Yeast Extract) Agar is used for the isolation and cultivation of Legionella species. II PERFORMANCE TEST PROCEDURE 1. Inoculate representative samples with the cultures listed below. a. Add 0.1 mL of a culture containing 30–300 CFU/0.1 mL to each plate and spread-inoculate using a sterile glass spreader. b.
Jan 02, 2020 · Buffered Charcoal Yeast Extract (BCYE) Feeley et al in 1978 developed a medium to isolate Legionella species which was later modified by substituting yeast extract for casein hydrolysate and beef extract, and replacing starch with activated charcoal and named it as Charcoal Yeast Extract (CYE) Agar. A further modification was made by Pasculle The new medium, designated BCYE for Buffered Charcoal Yeast Extract, could also be incubated aerobically. In 1981, Edelstein et al. increased the sensitivity of the medium by adding the potassium salt of alpha-ketoglutaric acid. Pretreatment with an acid wash and inoculation onto selective buffered charcoal-yeast extract agar (as formulated for the detection of Legionella spp.) can increase the recovery of Nocardia from respiratory specimens. 38 Although uncommon, N. asteroides complex strains can be isolated in automated blood culture systems, especially if patients Legionella Agar Buffered Charcoal Yeast Extract (BCYE), 90mm Plate. A non-selective medium for the maintenance of Legionella, spp. Product Code: 8065